Osmosis in Different Concentrations

I am going to investigate osmosis when tater is turn upd in distinguishable immersions of saccharose. I am aiming to witness osmosis in 5 assorted compactnesss of saccharose. I bequeath persona 5 varying concentrations so that I shake a wider spread to comp ar the results, and check that I dont stool any anomalies Prediction Osmosisis the handle of diffusion of piss system molecules from a weaker solvent into a stronger declaration, through a articulated lorrypermeable tissue layer.The footling pores in the membrane of the white murphyes entrust al down in the mouth the piss molecules to go in and out of the stump spud cadre, depending on the concentration gradient in the midst of the potato and the sucrose solution. If the piss supply concentrationis lour in the potato than in the sucrose solution, and so water result pass from the sucrose solution into the potato, and it allow for gain fish.If at that protrude is a full(prenominal)er concentration o f water in the potato, then the water volition go out of the potato and into the sucrose solution, as osmosis is the movement of water molecules from a region of high concentration to a region of low concentration, through a semi permeable membrane. So, I predict that the higher the concentration of sucrose, the lower the charge of the potato as water from the potato bottoms into the sucrose solution. Predicted graph of results IMAGE implement * 6 test tubes * 6 different concentrations of sucrose solutions * stump spud Potato cutter/core * Digital measuring homes * Measuring cylinders * Knife * analyse tube racks * Tissue paper Method I ordain take on 1 piece of potato in each tube. As in that location argon 15 tubes I pull up stakes scram to calculate how numerous pieces I lead need alto acheher. I think this would be a suitable calculation 115=15. So, I will stuff out many pieces of a potato using a core, and then cut 15 firearms with a knife. Then I will visor 10ml of each of the 6 sucrose solutions using a measuring cylinder. I will be given these different hard solutions in the popular laboratory.I will label the tubes 1 to 15 and fill each champion with 10ml of the solution. thermionic tube 1-3 will throw a concentration of 0 Tube 4-6 will have a concentration of 0. 4 Tube 7-9 will have a concentration of 0. 8 Tube 10-12 will have a concentration of 1. 2 Tube 13-15 will have a concentration of 1. 4 I will quantify each cut of meat on the measuring exceeds to identify each potato slice with its pack before and after immersion in the sucrose. Having respectd each slice on the scale and legered its weight unit, I will then authority the slices in the test tube containing the solution for 24 hours.Then I will empty the solution from the test tube by pouring it in the sink and remove the potatoes by hand and place them one by one on the scale. For accuracy, I will make sure that I wipe off any excess solution on the scale befo re placing each new slice on it. I will record the weight after placing them in the solution for 24 hours. This process will be repeated for tubes 1 to 15 and the results will be noned in a table and then plotted on a line graph. I will make this a amusement park test by only varying the concentration of sucrose and the surface of the potato slices, but keeping everything else the equal.The same potato must be use for the whole experiment or former(a)wise, the results would differ as the outmatchride and sizes might be different, which means one potato might have much water in it than the other. I will use a 10ml measuring cylinder so that I can accurately measure the exact amount of sucrose needed. As the cylinder measures different sucrose solutions, it has to be washed out each time I measure other 10ml of sucrose for the next tube, because it whitethorn be contaminated with the different sucroses. each(prenominal) the tubes will be unplowed for the same time, in the sam e place, so that the uncontrollable temperature would non affect the tubes separately. I will not be able to control * Temperature because I wont be in the laboratory for 24 hours, and the temperature could change in the night, or morning. *Temperature of solution- because I was not able to obtain a thermometer and I was not in the laboratory for 24 hours so it could change. * Weighing scales because these are digital and therefore, it produces results by itself. I will be able to control Concentrations of sucrose they are already measured * Time I will do the experiment, and herald back the next day at the same time, and promptly record the results *weight of the potato- I weighed the potato slices before the experiment. For safety in the laboratory, I will be very careful using the sharp knife which I will be using to cut the potatoes with. I will make sure that I have an overall so that my change state dont get dirty if the solutions spills. I will opine not to put potato es or sucrose in my mouth as they might have been infected by chemicals in the lab, which are poisonous.I will measure the weight of the potato in grams, and the concentration of sucrose in percentage. I am expecting everything to work out well as I have a perfectly good method but if I feel that the results may be wrong, I will repeat my experiment. earlier results These are my results This experiment place uprights my plan and prognostic. The carrot gains weight in rule water, and decreases weight in hard sugar solutions. By doing this experiment, I conceive that my method is good, and it will work. I think I should specifically be aware of the scales, because they alter a lot.However, this was carried out at home, so the cooking scales may have not been so accurate, as accuracy is not very important in cooking. Results these are my first results. They proved to be wrong. This mistake would have been make my human. I assume that I used two different scales, and have got all the weights jumbled up. The weight interpreted at the beginning is also not correct, so I may have made a mistake right from the start. Concentration of sucrose. pack before Weight after 0 1. 28 0 1. 33 0 1. 29 0. 4 1. 29 0. 4 1. 27 0. 4 1. 2 0. 8 1. 31 0. 8 1. 3 0. 8 1. 3 1. 2 1. 29 1. 2 1. 37 1. 2 1. 27 1. 6 1. 26 1. 6 1. 3 1. 6 1. 31 Conclusion I found out that as the concentration of sucrose increases, the weight of the potato decreases. In water it gained 1. 26 grams, but in 1 saccharose solution, it lost 0. 16 grams. My prediction supports my conclusion. I have calculated the bonnie change in weight to have a simple, clear creative thinker where the experiment is leading me to. Instead of having a large number of weights, I combined them into one by averaging them.I found the average by adding the weight gain/loss for each tube and divided it by three. To rule the percentage, I multiplied that decimal by 100. I drew a line graph, and then a line of best fi t, which is sloping downwards, negatively. This proves that the weight is getting lower as the percentage of the sucrose concentration is getting higher. In my investigation I found a definite relationship between the 2 variables weight and concentration of sucrose. Any increase in sucrose concentration led to a decrease in weight. The prediction is supported by the evidence of the graph.Although there is one anomaly, all the other results stand out and give a straight line of best fit exactly as I predicted. The point of early plasmolysis is where the concentration of sucrose and potato are even. at that place is no osmosis taking place at that point. As I said in my plan, if the water concentration is lower in the potato than in the sucrose solution, then water will pass from the sucrose solution into the potato, and it will gain weight. If there is a higher concentration of water in the potato, then the water will go out of the potato and into the sucrose solution.This is bec ause osmosis is the movement of water molecules from a region of high concentration to a region of low concentration, through a semi permeable membrane. The up raise of sucrose is the downfall of potato mass. IMAGE Osmosis In osmosis, water diffuses through a semi-permeable membrane. This diagram illustrates the concentrated sugar solution, separated from dilute sucrose solution by a selectively permeable membrane. This has pores (holes) in it which are very abject, and selects what it wants to allow through i. e. small molecules.Water molecules are very small. Each one is made of two hydrogen atoms and one oxygen atom. However, sugar molecules are some(prenominal) times larger. In potatoes, the pores of the membrane only let the water through. There is a higher concentration of sugar molecules on the right-hand place of the membrane in the diagram, than in the left-hand side. Sugar molecules would diffuse from the concentrated solution into the dilute one until they were evenl y spread out if there was no membrane, but they cannot do this as the pores are too small for the sugar to get through the membrane.Therefore, the small water molecules diffuse into the concentrated sugar solution, to make it more dilute. This process is called osmosis. It is the diffusion of water molecules from a place where they are in a higher concentration, to a place where they are in a lower concentration, through a selectively permeable membrane. Potato cells plasmolyse in concentrated solutions. This diagram illustrates a establish cellIMAGE (which is similar to a potato cell) in a concentrated solution. It will lose water by osmosis. The cytoplasm and the vacuole will shrink.The cell membrane is semi-permeable and the vacuole contains a sucrose solution. So when a cell is placed in distilled water (high concentration) water will move across the semi-permeable membrane into the cell (lower water concentration) by osmosis, making the cell swell. This cell is called turgid. In potato cells, the cells would increase in length, volume and weight because of the extra water in the potato. If the potato was to be placed in a lower concentration, then the opposite would happen, because water would move out of the cell into the solution.If the solution is very concentrated, then a lot of water will diffuse out of the cell. The cytoplasm and vacuole will keep shrinking, but the cell palisade will not as it is too stiff. As the cytoplasm shrinks pass on and further into the centre of the cell, the cell wall gets left behind. The cell membrane, contact the cytoplasm, tears away from the cell wall. If this happens, the cell is said to be plasmolysed. The potato will therefore, decrease in length, volume and weight. Plasmolysis is the point where the membrane is altogether detached from its ell wall, and the potato is killed. Evaluation my experiment shows some accurate results. It concludes the experiment, and proves my prediction. My final results were quit e reliable due to the precautions I took to make thisa bonny test. The graph has a straight slope pointing downwards, which is the clearest way to understand my prediction. All of them are not that closely together, neither far away, so a line of best fit joins some tips of the points, and causes it to go straight down.I have one anomalous result, which falls on 0 on the x axis. This occurred in tube 5, which contained a concentration of 0. 4. Perhaps I did not carry out that properly. These were the main problems in carrying out the experiment- * Scales kept moving * Difficult to get out the potato from the core * Potatoes get stuck in tube. * Solution is still left after wiping the potato and the scales * variant scales * couldnt control the temperature the point of early plasmolysis would not be valid.This is because I have just plotted it on the graph on a line which suits my results. To prove that it is the right point, I would have to do another experiment to find that out. The other evidence is likely to be valid, because as all the evidence links to the results. My investigation is fair because I did not change many things, expect the concentration of sucrose. I think that I have collected quite a lot of evidence to support my conclusion. The result table is the main source of evidence.I could try investigating with more percentages of sucrose concentration, the size of the potato and have more short intervals between the sucroses. This would have a more spread to the results, and therefore, results can be compared and evaluated more thoroughly. They would be very accurate as well as reliable. As I have an anomaly, my evidence may not be extremely correct. I would have to do more research to what I have already done (in the conclusion) on osmosis, and contact what actually happens, and see if it occurs in plant cells all the time.If I were to repeat the experiment, I would use a very accurate scale, so that results would come out accurately and then I would have a accurate average. The graph will be fairly accurate. I would also make sure that the scale and the potato slices are properly wiped. This is because if they are not wiped, the weight would increase, and will not be accurate. I could use a syringe or a burette to measure the 10ml of sucrose solution, because those two apparatus are very accurate.I would do several more experiments with bigger sizes of potatoes to obtain more evidence to support my conclusion. I will also do some research on osmosis, and this will make me certain of my evidence if I have many to compare with. I will agree with the majority. Overall, I am very pleased with these results and with the evidence I have so far, that osmosis occurs when there is a high concentration and a low concentration, some(prenominal) aside a semi-permeable membrane. The lower concentrated substance diffuses through the membrane to where there is the higher concentrated substance.